Figure 2.
ERRγ inhibits the growth of MDA-MB-231 xenografts. A, Tumor growth rates in SCID mice inoculated orthotopically with MDA-MB-231 populations expressing control (EGFP), PGC-1α or ERRγ. Data are tumor volumes measured at indicated days after injection and represent mean ± SEM from one of two representative experiments (n=8 to 9). B, Tumor weights at day 63, displayed as scatter plots with median values. C, Protein expression of transgenes in indicated tumors, detected by western blot analysis. D, Induced expression of ERR target genes in MDA-PGC-1α and MDA-ERRγ tumors. Data are expressed relative to mRNA levels of each gene in control tumors and are the mean ± SEM of 14 tumors for control, 12 tumors for PGC-1α and 6 tumors for ERRγ. n.s., not significant; *, P < 0.05; **, P<0.01; ***, P<0.001.