Figure 5.
MDA-ERRγ tumors display markers of MET. A, Levels of mRNA for epithelial [E-cadherin (CDH1), mucin 1 (MUC1), keratin 8 (KRT8)] and mesenchymal [vimentin (VIM)] markers in control, PGC-1α and ERRγ tumors, determined by RT-qPCR and expressed as in Fig. 2D. *, P < 0.05; **, P<0.01. B, E-cadherin protein levels in three MDA-control and three MDA-ERRγ tumors, detected by western blot analysis. Ponceau staining of the blot (lower panel) is shown as loading control. C,D, Immunohistochemical staining of E-cadherin (C) and vimentin (D) in sections of 3 tumors from each group.