Figure 6.
ERRγ decreases the invasiveness of MDA-MB-231 cells, partly via induction of E-cadherin expression. A, Decreased invasiveness of MDA-PGC-1α and MDA-ERRγ cells relative to MDA-control cells. B, E-cadherin knockdown by shRNA partially restores the decreased invasiveness of MDA-ERRγ cells. A & B, Data are expressed relative to the number of matrigel-invading MDA-control cells (set equal to 100; 1907 cells in panel A, 1085 cells in panel B) and are the mean ± SEM of 3 (A) or 2 (B) experiments performed in triplicate. C, E-cadherin and ERRγ protein expression in cells of panel B, determined as in Figures 1A and 3C. Ponceau staining of the blot (lower panel) is shown as loading control.