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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Cancer Res. 2011 Feb 15;71(7):2433–2444. doi: 10.1158/0008-5472.CAN-10-1875

Figure 1. Methods of Controlled Heterotypic Cell Co-Culture Allow Analysis of the Cell-Cell Communication Phenotype.

Figure 1

(A–D) RFP-HUVEC and GFP-1205Lu metastatic melanoma cells were co-cultured in colonies separated by a precisely defined gap (method I), in random mixtures (method II) or with an RFP-HUVEC colony of precisely defined size and shape surrounded by GFP-1205Lu cells (method III); the phenotypes of cells co-cultured using method I (B), method II (C) and method III (D) were determined at 6 hrs. (upper panels) and 48 hrs. (lower panel).

(E–H) RFP-HUVECs alone were cultured for 48 hrs. in EGM-2 medium (E) or in conditioned medium (CM) from HUVECs (F), 1205Lu cells (G), or co-cultured RFP-HUVEC and GFP-1205Lu cells (H).