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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Feb 21;407(1):34–38. doi: 10.1016/j.bbrc.2011.02.087

Figure 2.

Figure 2

(A) FEN1 mutations used in this study. All contain myc-tag at the N-terminus. FL: full length Myc FEN1. ΔC, ΔP, ΔPΔC and ΔPΔC +NLS represent deletions in the C terminus and PCNA binding regions. E160D and FFAA (F343A/F344A) represents single amino acid substitution mutants. (B) Myc-FEN1 along with FLAG-hTERT were co-transfected to 293T cells and co-IP was performed with anti-Myc antibody followed by anti-FLAG western blotting for detecting FLAG-hTERT. As negative controls single transfection of either FLAG-hTERT or pCl-neo empty vector (top panel lanes 3 and 4) were used. IP Input for both FLAG-hTERT and Myc-FEN1 (bottom panels) represents 20% of the total protein used in immunoprecipitation. Representative images from at least 4 independent experiments are shown.