Skip to main content
. 2011 Apr;25(7):701–716. doi: 10.1101/gad.2002611

Figure 1.

Figure 1.

SKIP is required for DNA damage-induced p21 gene expression. (A) qRT–PCR analysis of p21 (top panel) and PUMA (botom panel) mRNA levels. U2OS cells were transfected with control or SKIP siRNA for 48 h, and incubated in the presence or absence of etoposide (20 μM) for the indicated times. (Right panel, lanes 1–6) Protein lysates were subjected to immunoblot analysis. (B) qRT–PCR analysis of p21 (top panel) and PUMA (bottom panel) mRNA levels. HCT116 cells were transfected with control or SKIP siRNA for 48 h, and incubated in the presence or absence of doxorubicin (0.5 μM) for the indicated times. (Right panel, lanes 1–6) Protein lysates were subjected to immunoblot analysis. All of the mRNA expression levels were normalized to GAPDH mRNA, and the values represent the fold increase or decrease over untreated cells. Error bars represent the standard deviation obtained from three independent experiments.