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. 2011 Apr;25(7):701–716. doi: 10.1101/gad.2002611

Figure 2.

Figure 2.

Loss of SKIP does not affect p21 gene transcription. (A) Schematic representation of the p21 gene locus, and the relative locations of the primers used for ChIP. (B) ChIP analysis in U2OS cells transfected with control or SKIP siRNA for 48 h, followed by vehicle or etoposide (20 μM) for a further 6 h. ChIP-enriched DNA was quantified by qPCR with the indicated primers, and values are expressed as percentage of input DNA. Error bars represent the standard deviation obtained from three independent experiments. (C) qRT–PCR analysis of p21 and PUMA primary transcripts. U2OS cells (top panel) or HCT116 cells (bottom panel) were transfected with control or SKIP siRNA, and incubated with etoposide (top panel) or doxorubicin (bottom panel) for the indicated times. Numbers of the primers indicate the position of the first base pair relative to the transcription start site. The mRNA expression levels were normalized to GAPDH. Error bars represent the standard deviation obtained from three independent experiments.