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. 2010 Nov 3;30(44):14786–14794. doi: 10.1523/JNEUROSCI.4124-10.2010

Figure 1.

Figure 1.

Tissue and developmental distribution of MINK/TNIK proteins. A, Western blot analysis of postnuclear supernatants isolated from adult rat tissues. Molecular mass is indicated in kilodaltons. B, Developmental regulation of MINK and TNIK. Postnuclear supernatant Western blot analyses of hippocampus and cortex isolated from embryonic, postnatal and adult rat tissues. E18, Embryonic day 18; P3, P5, P8, postnatal day 3, 5, and 8, respectively; Ad, adult. C, MINK and TNIK are enriched in PSD. Western blot analyses of PSD subcellular fractionation from rat brain. S1, P1, Whole-brain postnuclear supernatant and membrane fractions, respectively; S2, cytosolic proteins; P2, crude synaptosomal fraction (20 μg each); PSD, Triton X-100-extracted PSD (5 μg of protein). Antibodies for immunoblotting are indicated to the right of panels. D, Subcellular distribution of MINK/TNIK in hippocampal neurons. Confocal microscopy of 23-d-old hippocampal neurons stained for MINK/TNIK, phalloidin, and bassoon. Arrows indicate colocalized puncta. Box in “merge” panel indicates region of higher magnification. Scale bars, 20 μm.

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