Figure 4.

Insults on P10 did not alter neural production. (A) A confocal image showing proliferating progenitors expressing Ki67 (red) and newborn GCs expressing POMC–GFP (green) from a P17 mouse. There was no co-localization between Ki67 and POMC–GFP in any condition, consistent with the idea that POMC–GFP labels postmitotic newborn neurons. Scale bar, 50 μm. (B) Propidium iodide (PI) was used as a non-specific nuclear stain to quantify the total number of cells in the granule cell layer. Scale bar, 100 μm. (C) Stereological quantification revealed no differences in the number of POMC–GFP-, Ki67-, or PI-labeled cells on P17 between treatment groups (p > 0.05; ANOVA). For POMC–GFP labeling, n = 8, 9, and 9 mice per group, respectively. For Ki67 labeling, n = 3, 4, and 4, respectively.