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. Author manuscript; available in PMC: 2011 Apr 5.
Published in final edited form as: Nat Med. 2010 Mar 28;16(4):429–437. doi: 10.1038/nm.2099

Figure 3.

Figure 3

Insulin increases nuclear transport of XBP-1s. (a) Nuclear and cytoplasmic protein amounts of XBP-1s, p85α and p85β in MEFs infected with a constant dose of XBP-1s and p85α-Flag and increasing doses of p85β-HA. (b) Nuclear and cytoplasmic protein amounts of XBP-1s, p85α and p85β in MEFs infected with a constant dose of XBP-1s and p85β-HA and increasing doses of p85α-Flag. (c) HA and SH2 blotting in Flag immunoprecipitates of cells infected with Ad-p85α-Flag and Ad-p85β-HA. (d) HA and Flag immunoblotting in p85α-Flag immunoprecipitates after insulin (500 nM) stimulation. (e) Nuclear protein amounts of XBP-1s in insulin (500 nM)-stimulated XBP-1s–infected MEFs. (f) mRNA levels of p85α and p85β in the p85α and p85β DKD cells. (g) Protein amount of p85 in the DKD cells. (h) Nuclear and total protein levels of XBP-1s in DKD cells that were infected with Ad-XBP-1s and stimulated with insulin for 10 and 15 min. (i) XBP-1 splicing assay in PLKO and DKD cells that were stimulated with tunicamycin (0.75 μg ml–1) for the indicated time periods. (j) Nuclear and total XBP-1s protein amount in PLKO and DKD cells after tunicamycin (2 μg ml–1) stimulation. Each experiment was independently repeated three times.