Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primer extension analysis of the virulence plasmid-encoded icsP gene (2457T), the virB mutant (AWY3), the icsP inducible plasmid (pHJW6), and the icsP-lacZ promoter fusion (pHJW20). The new TSS, P2, is identified with its relative location to the annotated TSS, P1. A sequencing reaction (first four lanes) was used to calibrate the gel. The experiment was repeated three times and representative data are shown.
