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Activity of the icsP promoter constructs in response to growth phase. pHJW20 is a construct carrying both promoters, pKLM03 carries the P2 promoter alone, pCTH03 carries neither icsP promoter. Promoter activities were measured throughout growth (0–10 h) and optical densities of the cultures carrying each promoter construct are shown. The data is normalized using pMIC21, the promoterless lacZ gene. Assays were run in triplicate and the means and standard deviations are shown.
