Table 2.
Contribution of P2 promoter activity to total icsP promoter activity
Environmental parameter | Specific condition in Luria-Bertani medium | Percent contributiona |
---|---|---|
pH | 7.4 | 75.54 ± 6.96 |
5.5 | 61.88 ± 19.55 | |
Iron concentration | Normal (0 µg ml-1 EDDA) | 69.86 ± 1.12 |
Reduced (15 µg ml-1 EDDA) | 74.81 ± 5.48 | |
Osmotic pressure | Normal NaCl concentration (LB) | 72.32 ± 0.07 |
Half NaCl concentration (LO) | 67.20 ± 3.96 |
Average percent contributions are based upon the promoter activity of P2 (pKML03) compared to the total promoter activity (pHJW20). Assays were run in triplicate and the means and standard deviations are shown.