Table 2.

Physical and potassium release properties of liposomal AmB formulations prepared from DSHemsPC using DSSH buffer pH 6.5

AmB Formulation	Molar ratio	Zeta average size (nm) ± SD	Distribution	Polydispersity ± SD	Zeta potential (mv) ± SD	IC50 for potassium release (μg/ml, Lower and Upper 95% limit)
F21-DSHemsPC/DSPC/DSPG/AmB	1.25/5.0/2.0/1.0	143.3±1.0	Bimodal	0.254±0.01	−33.4±4.8	81(22–310)
F22-DSHemsPC/DPPC/DPPG/AmB	1.25/5.0/2.0/1.0	99.36±2.5	Bimodal	0.21±0.13	−40.9±9.6	22360 (1500–33700)
F23-DSHemsPC/DMPC/DMPG/AmB	1.25/5.0/1.5/1.0	121.1±4.5	Monomodal	0.183±0.01	−42.2±5.5	6000 (290–125510)
F24-DSHemsPC/DMPC/DMPG/AmB	1.25/5.0/2.0/1.0	78.22±0.4	Monomodal	0.23±0.02	−24.9± 11.5	1970 (465–8340)
F25-DSHemsPC/HSPC/DSPG/AmB	1.25/5.0/2.0/1.0	97.07±0.7	Monomodal	0.187±0.03	−31±7.1	55 (8–355)
F26-DSHemsPC/HSPC/DSPG/AmB	1.75/5.0/1.5/1.0	169.3±0.4	Monomodal	0.288±0.04	−37.5±6.1	119 (34–420)
AmBisome™		120.3±1.5	Monomodal	0.2±0.2	−54.5±1.1	529.97 (326.6–1101.7)
Fungizone™						0.76 (0.41–1.4)

The IC50 of F21– F24, F26 and AmBisome™ was significantly (P < 0.01) higher than Fungizone™. The IC50 among F25 and Fungizone™ was not significantly different (P > 0.05). The IC50 of AmBisome™ was significantly (P < 0.001) greater than F21, F25, and F26. The IC50 of F22, F23, and F24 was significantly (P < 0.05–0.001) greater than AmBisome™.