Table 3.

Physical and potassium release properties of liposomal AmB formulations prepared from DSHemsPC using DSSH buffer pH 5.5

AmB Formulation	Molar ratio	Zeta average size (nm) ± SD	Distribution	Polydispersity ± SD	Zeta potential (mv) ± SD	IC50 for potassium release (μg/ml, Lower and Upper 95% limit)
F27-DSHemsPC/DSPC/DSPG/AmB	1.25/5.0/2.0/1.0	84.7±0.8	Monomodal	0.198±0.03	−23.3± 6.07	130 (16.7–990)
F28-DSHemsPC/DPPC/DPPG/AmB	1.25/5.0/2.0/1.0	202.4±20	Monomodal	0.5±0.03	−14.8±4.16	14710(599–361070)
F29-DSHemsPC/DMPC/DMPG/AmB	1.25/5.0/1.5/1.0	111.6±1.0	Monomodal	0.21±0.02	−25.3±9.05	1342000(307000–58729000)
F30-DSHemsPC/DMPC/DMPG/AmB	1.25/5.0/2.0/1.0	112.8±3.2	Monomodal	0.207±0.01	−25.3±13	114510(29560–443580)
F31-DSHemsPC/HSPC/DSPG/AmB	1.25/5.0/2.0/1.0	99.53±2.8	Monomodal	0.237±0.03	−21.3±7.97	50 (6.6–380)
F32-DSHemsPC/HSPC/DSPG/AmB	1.75/5.0/1.5/1.0	130.1±2.0	Monomodal	0.384±0.01	−24±5.13	217 (56–840)
F33-DSHemsPC/DMPC/DMPG	1.25/5.0/1.5	76.98±0.39	Monomodal	0.21±0.056	−42.7±0.5	Inactive
AmBisome™		120.3±1.5	Monomodal	0.2±0.2	−54.5±1.1	529.97(326.6–1101.7)
Fungizone™						0.76 (0.41–1.4)

The IC50 of F27– F30, F32 and AmBisome™ was significantly (P < 0.01) higher than Fungizone™. The IC50 among F31 and Fungizone™ was not significantly different (P > 0.05). The IC50 of AmBisome™ was significantly (P < 0.001) greater than F27, and F32. The IC50 of F29 was significantly (P < 0.001) greater than AmBisome™.