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. 2011 Apr;13(4):348–357. doi: 10.1593/neo.101580

Figure 5.

Figure 5

HCEC 1CT+7 displays defects in cell migration. (A) In a transwell migration assay, cells were seeded in serum-free medium and allowed to migrate through an 8-µm pore membrane toward either 2% or 0% serum medium. Migrated cells were stained with DAPI. A549 lung cells are used as a positive control for cell migration. (B) Quantification of (A) by averaging the number of cells per 20x magnification (n = 15). (C) HCEC 1CT+7 are slower to migrate into a gap created by an experimental wound to the cell monolayer. (D) Quantification of (C) by averaging the number of cells that migrated into the gap compared with 0 hours (n = 3). All columns represent mean ± SEM. **P < .005, by two-tailed, unpaired Student's t test.