Figure 2.

Gα₁₂ interacts with the C-terminal domain of JLP. (A) Schematic diagram representing JLP deletion mutant constructs encoding different domains. These constructs encode S-tagged JLP-domain I (amino acids 1–164), II (amino acids 165–473), IIΔ (amino acids 210–398), III (amino acids 465–1008), C (amino acids 997–1170), and IV (amino acids 1165–1307). (B) Vector constructs expressing Gα₁₂QL were coexpressed with the different domain mutants of S-JLP in COS-7 cells. After 48 hours, the lysates were subjected to the pulldown assay with the S-protein agarose beads. The coprecipitated Gα₁₂ was identified by immunoblot analysis using antibodies to Gα₁₂. (C) The expression levels of transfected Gα₁₂QL and JLP constructs were monitored by immunoblot analyses using antibodies to Gα₁₂ (upper panel) or S-tag (lower panel).