Figure 4.

Effect of JLP and GID-JLP on Gα₁₂QL-mediated foci formation. Parental NIH3T3 cells were transfected with empty vector pcDNA3 (VC), Gα₁₂QL, full-length JLP (FL-JLP), Gα₁₂QL + FL-JLP, GID-JLP, Gα₁₂QL + GID-JLP, or Gα₁₂QL + GID-JLP + FL-JLP using the calcium phosphate transfection method. (A) The representative foci formed at 14 days were stained photographed (4x). Results presented are from a typical experiment (n = 4). (B) The foci formed from four independent experiments were scored and plotted (mean ± SD).