Figure 5.

Effect of JLP and GID-JLP on Gα₁₃QL-mediated foci formation. (A) NIH3T3 cells were transfected with empty vector pcDNA3 (vector), full-length JLP (FL-JLP), GID-JLP, Gα₁₃QL, Gα₁₃QL + FL-JLP, Gα₁₃QL + GID-JLP, or Gα₁₃QL + GID-JLP + FL-JLP using the calcium phosphate transfection method. After 14 days, the number of foci from three independent experiments was scored and plotted (mean ± SD). (B) At 14 days after transfection, expressions of the encoded proteins by the transfected complementary DNA constructs were verified by immunoblot analysis using a parallel set of transfectants.