Figure 2.

The antitumor effect of CIM is reversed by the adenosine A₃ receptor antagonist MRS1191. (A) A₃R RNA expression on melanoma tissue (lanes 1, 2, 3, and 4 indicate four different samples harvested from melanoma-bearing mice). (B) MRS1191 (24 µg/kg, black bars) was administered by i.p. injection with PBS or CIM 1 µg/kg (n = 10). The effect of CIM on melanoma growth as measured by tumor volume was significantly reversed by MRS1191. (C) MRS1191 significantly reversed the effect of CIM on the number of proliferative (BrdU⁺) cells (n = 5). Data are expressed as mean ± SEM. Statistical differences were determined by Student's t test.