Figure 5.

Tumor-infiltrating DCs are mature and active in mice treated with CIM. (A) The number of myeloid DCs (mDCs, CD11c⁺ CD11b⁺ cells) significantly increased in melanoma tissue of mice treated with CIM compared with PBS (n = 5). Tumor-infiltrating mDCs were mature and active as indicated by increased expression of the maturation marker MHC class II (B) and activation marker CD80 (C), respectively (n = 5). Splenic CD11c⁺ cells isolated from naive mice and stimulated with CIM (20 nM-200 nM-2 µM) in vitro for 24 hours released higher amounts of TNF-α (D) and IL-12p40 (E) than PBS-stimulated cells (n = 12). All data are expressed as mean ± SEM. Statistical differences were determined by one-way ANOVA followed by Dunnett post hoc analysis.