Figure 6.

The antitumor effect of CIM is mediated by CD8⁺ T cells. (A) CD3⁺CD8⁺ T cells were significantly increased in melanoma tissues of mice treated with CIM compared with PBS (n = 10). (B) Representative FACS plots are shown in the right panel. (C) The antitumor effects of CIM (1 µg/kg) was significantly reversed in CD8⁺ T-cell-depleted mice (black bar) compared with IgG controls (open bar) (n = 7). Depletion of CD8⁺ T cells (black bars) significantly reduced CIM-induced TNF-α (D) and IFN-γ (E) expression in melanoma tissue homogenates (n = 7). All data are expressed as mean ± SEM. Statistical differences were determined by either two-tailed Student's t test or one-way ANOVA followed by Dunnett post hoc analysis as appropriate.