PBS:
Sodium phosphate, dibasic (Na2HPO4)	1.44 g
Sodium chloride	8 g
Potassium phosphate (KH2PO4)	0.24 g
Potassium chloride	0.2 g
Distilled water	800 ml
Adjust pH to 7.4, and adjust to a final volume of 1 liter with additional distilled water.
PBS-T (PBS/Tween Solution):
0.1% volume of Tween 20 prepared in PBS
Triton X-100 (octylphenolpoly(ethyleneglycolether):
Roche Diagnostics, Cat. No. 11332481001.
Enzyme Reagent:
TdT (Roche Diag., Cat. No. 03333574001)	4 µl
TdT storage buffer	100 µl
Mix well and store at −20 °C.
Label Reagent:
Biotin-16-dUTP (Roche Diag., Cat. No. 11093070910)	4 µl
TdT reaction buffer	1 ml
Mix well and store at −20 °C.
TdT Reaction Mixture:
Enzyme reagent	100 µl
Label reagent	900 µl
Mix just before use. Use the remaining 100 µl of label solution as a negative control.
Stop Wash Buffer:
NaCl (MW: 58.44)	1.75 g
Sodium citrate, trihydrate (MW: 294.11)	0.88 g
Distilled water	100 µl
Mix to dissolve and store at room temperature.