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. Author manuscript; available in PMC: 2011 Apr 5.
Published in final edited form as: Cell Death Differ. 2009 Jul 3;16(10):1408–1417. doi: 10.1038/cdd.2009.86

Figure 4.

Figure 4

Cyclin D1 interacts with FOXO1 in vitro and in vivo. (a) Interaction of endogenous cyclin D1 and FOXO1 proteins in LNCaP cells as shown by co-immunoprecipitation using an antibody against cyclin D1. (b) LNCaP cells were transfected with plasmids as indicated. At 48 h after transfection, cells were subjected to immunoprecipitation and western blot analysis. (c) Binding of 35S-labeled cyclin D1 proteins, produced by in vitro transcription and translation, to GST–FOXO1 recombinant proteins. Top, autography of in vitro-produced cyclin D1 proteins bound by GST–FOXO1 recombinant proteins. Middle, input of 35S-labeled cyclin D1 proteins. Bottom, GST and GST–FOXO1 recombinant proteins used for in vitro protein-binding assays indicated by Coomassie blue staining