Table 2.

Methods used by participating laboratories for detection of XMRV nucleic acids in plasma specimens

Lab	Extraction method	Volume plasma (µl)	Assay #	Target	Assay type	Quant/ Nested	Primer Reference
CDC	Ultracentrifuge/ QIAamp viral RNA mini	500	1	pro	qRT-PCR	Quant	15
			2	gag	RT-PCR southern	Nested	9
FDA (H)	Qiagen viral RNA mini	140	1	gag	RT-PCR	Nested	2
FDA (Lo)	Ultracentrifuge/ TRIzol	250	1	gag	RT-PCR	Nested	4
			2	gag	RT-PCR	Nested	4
GP	Magnetic- based target capture	500	Duplex	Proprietary	TMA	N/A	Unpublished
NCI	Ultracentrifuge including internal standard Guanidinium Isothicyanate	~200– 500ul plasma	1	5’ UTR of gag	RT-PCR SCA	Quant	Unpublished
WPI	QIAamp viral RNA mini	140	1	5’ UTR of gag	qRT-PCR	Quant	24