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. 2010 Dec 21;203(4):537–544. doi: 10.1093/infdis/jiq070

Figure 2.

Figure 2.

Activation of MAPK is essential for the production of IL-8 induced by V. parahaemolyticus. A, Caco-2 cells were infected with V. parahaemolyticus for the indicated times. After infection, the cells were lysed and normalized for protein content. Activation of MAPKs (ERK1/2 and p38) was analyzed by Western blotting with anti-phospho-p44/42 (P-ERK1/2) and anti-phospho-p38 (P-p38) specific antibodies. The blots were stripped and re-probed with anti-p44/42 (ERK1/2) and anti-p38 (p38) antibodies. B, Caco-2 cells were treated with the MAPK inhibitor UO126 (10 μM) or SB203580 (30 μM) for 60 minutes prior to infection. IL-8 production was measured by enzyme-linked immunosorbent assay (ELISA). C, D, Caco-2 cells were infected with wild type V. parahaemolyticus (WT); mutant strains ΔtdhAS, ΔT3SS1 (ΔvcrD1), ΔT3SS2 (ΔvcrD2), ΔVP1680, ΔVP1686, ΔVPA0450, pT3SS1 (expressing plasmid encoded vcrD1 into ΔvcrD1); or pVP1680 (expressing plasmid-encoded VP1680 into ΔVP1680). Three hours after infection, the cells were lysed and normalized for protein content. Activation of ERK1/2 and p38 MAPK was analyzed by Western blotting. Data are means ± SD of 3 independent experiments with assays in duplicate. Statistical significance: *, P < .05; **, P < .01 compared with 6 hour-VP.