Figure 6.

.P-p38 MAPK stabilizes IL-8 messenger RNA (mRNA). A, Caco-2 cells were infected with V. parahaemolyticus for 6 hours to allow transcription of IL-8 mRNA. B, Infected Caco-2 cells were then treated with gentamicin (100 μg/mL), actinomycin D (5 μg/mL), and p38 inhibitor SB203580 (30 μM) or DMSO for the indicated times. Following inhibitor treatment, RNA was extracted and reverse transcribed by LightCycler polymerase chain reaction (PCR) to examine IL-8 mRNA stability. IL-8 mRNA was normalized with the 18S ribosomal RNA housekeeping gene. The results are presented as the relative decrease in stabilization of 18S ribosomal RNA. Data shown are the means ± SD of 3 independent experiments with assays in duplicate.