Table 1.

Primers, annealing temperatures and dispension orders used for amplification and sequencing of the different mutations

Mutation (gene)	PCR primer pair	Sequencing primer	PCR annealing temperature	Dispension order
rs5743305	F: 5′-GTAGCCCTGAGCCCAGTAACTATA-3′	5′- TGAGCCCAGTAACTATAAAG-3′		TCGTAGCTA
(TLR3)	R: 5′-BIOTIN-CCTAAGAACATGCCACAAGCCACCA-3′	(forward direction)	60°C
rs3775291	F: 5′-TCATTAAGGCCCAGGTCAAG-3′	5′- TTATTCTTGGTTAGGTTGA-3′		GAGTATGT
(TLR3)	R: 5′-BIOTIN-TGGCTAAAATGTTTGGAGCA-3′	(forward direction)	55°C
rs10774671	F: 5′-CCGTAAATGCTCACTGAATCC-3′	5′-TCATGTGTCTCACCCTT-3′		GAGTATGT
(OAS1)	R: 5′-BIOTIN-TGCAGGTCCAGTCCTCTTCT-3′	(forward direction)	60°C

NOTE. PCR, polymerase chain reaction.