FIG. 1.

Effects of DES exposure in the neonatal uterus. Pups were given control corn oil (CO) or DES injections daily from P1 to P5 (n = 5 per treatment per day). Uterine tissues were collected at the end of treatment and examined for alterations in histology, proliferation, and hormone receptors at the indicated postnatal time points. A) Uterine histology assessed using hematoxylin-and-eosin staining. B) Cell proliferation was detected by incorporated BrdU immunohistochemistry. Immunoreactive ESR1 and PGR were detected using specific antisera against ESR1 (C) and PGR (D). M, myometrium; S, stroma. Bars = 100 μm.