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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Biomaterials. 2011 Jun;32(16):3977–3987. doi: 10.1016/j.biomaterials.2011.02.021

Figure 2.

Figure 2

Multinucleate cell characterization. A) Percent nuclei found in multinucleate giant cells, B) percent multinucleate giant cells found in cell population, C) number of multinucleate giant cells per frame, and D) average number of nuclei per giant cell for primary- and secondary-derived fibroblasts and macrophages alone (short-term, 1–3 days, and long-term, 30 days) and in co-culture (short-term, 1–3 days) without the addition of exogenous cytokines. So few giant cells per frame in the long-term fibroblast culture condition is due to the increase in size of those fibroblasts compared to those cultured for short-term, consequently decreasing the overall number of cells per frame (see Figure 7). Data represent the mean ± SEM from 3 independent replicates. Significance (p < 0.05) was analyzed between primary and secondary fibroblast short-term monocultures and short-term co-cultures and between primary and secondary fibroblast short-term monocultures and long-term monocultures. Images were taken with a 15X objective and yielded a frame size of 260, 891 µm2.