Table 1.

Proteomic studies on mature human spermatozoa

Authors	Study	Proteomic Technique	Outcome
Johnston et al. 2005 [37]	First extensive analysis of human sperm proteome	LC-MS/MS	1760 proteins identified
Martinez-Heredia et al. 2006 [36] de Mateo et al. 2007 [40]	Goal to provide a reference 2D map of the mature human sperm proteome	MALDI-TOF	131 proteins identified
Ficarro et al. 2003 [38]	Phosphoproteome analysis of capacitated human sperm	LC-MS/MS	60 proteins identified
Domagala et al. 2007 [32]	Identification of sperm immunogenic antigens	LC-MS	35 proteins identified
Secciani et al. 2009 [33]	Protein profile of capacitated versus ejaculated human sperm	MS/MS	116 proteins identified
Shetty et al. 2001 [39]	Identification of immunocontraceptive candidates	MALDI-MS	8 proteins identified
Bohring et al. 2001 [34]	Isolation and identification of sperm membrane antigens	MALDI-MS	18 proteins identified
Baker et al. 2007 [29]	Identification of proteins in human sperm using LC-MS/MS analysis	LC-MS/MS	1056 proteins identified
Lefievre et al. 2007 [35]	Identify targets for S-nitrosylation in human sperm	LC-MS/MS	240 proteins identified
Li et al. 2007 [28]	Establish high-resolution 2-D map of human sperm proteins	MALDI-MS	16 proteins identified