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. 2011 Feb 17;103(7):571–584. doi: 10.1093/jnci/djr029

Figure 7.

Figure 7

Effect of phenethyl isothiocyanate (PEITC) administration on clusterin protein expression in plasma of mice transgenic for prostate adenocarcinoma. A) Clusterin levels in the plasma of mice fed control or 3 μmol PEITC/g diet. Results shown are mean plasma clusterin (ng/mg protein) with 95% confidence intervals (error bars) (n = 7 for mice fed the control diet and n = 8 for mice fed the 3 μmol PEITC/g diet). *P = .02 (two-sided mixed-effects analysis of variance). B) Detection of clusterin protein by western blotting of cell lysates and conditioned media from TRAMP-C1 cells treated for 24 hours with the indicated concentrations of PEITC. Actin was used as a loading control. Bar graph shows mean (arbitrary units) and 95% confidence intervals (error bars). (n = 4 from lysates prepared from three different batches). *P ≤ .05 (two-sided Student t test). C) Detection of clusterin protein levels by western blotting of cell lysates and conditioned media from TRAMP-C1 cells treated for 24 hours with 10 and 20 μM D,L-sulforaphane (SFN), 2.5 and 5 μM allyl isothiocyanate (AITC), 2.5 and 5 μM benzyl isothiocyanate (BITC), 20 and 40 μM diallyl trisulfide (DATS), and 2.5 and 5 μM gluconasturtiin (GN). Actin was used as a loading control. Bar graph shows mean (arbitrary units) and 95% confidence intervals (error bars) (n = 5 from lysates prepared from two different batches). *P ≤ .05 and **P ≤ .001 (two-sided Student t test).