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. 2011 Apr 5;9(4):e1001040. doi: 10.1371/journal.pbio.1001040

Figure 2. Accumulation of NDH subcomplex A was impaired in crr27.

Figure 2

(A) A schematic model of the NDH-PSI supercomplex in chloroplasts. The NDH complex is divided into four subcomplexes and interacts with at least two copies of PSI to form the NDH-PSI supercomplex corresponding to Band I, which can be detected by BN-PAGE (B). Eleven plastid-encoded subunits are depicted by green letters; nucleus-encoded subunits are depicted by red letters. Subcomplex A, surrounded by the red line, is missing in the sub-NDH-PSI supercomplex corresponding to Band II, which is detected in crr27 and ndhl mutants (B). (B) BN-PAGE analysis of thylakoid protein complexes. After electrophoresis, the gel was stained with CBB. The complexes are identified in the original non-stained gel in Figure S3A. (C) Immunoblot analysis of the thylakoid proteins from various genetic backgrounds with the indicated antibodies. Thylakoid proteins were loaded on an equal chlorophyll basis, and the series of dilutions is indicated.