Table 1. Summary of the chaperonin and NDH subunits detected in the Cpn60β1 and Cpn60β4 IP fractions.
Protein Name | Cpn60β1 IP | Cpn60β4 IP | emPAI Ratioa | ||||
Mowse Score | Protein Match | Coverage (%) | Mowse Score | Protein Match | Coverage (%) | ||
Cpn60α1 | 2,177 | 64 | 69 | 6,369 | 188 | 72 | 3.9 |
Cpn60β1 | 6,794 | 230 | 70 | 5,591 | 202 | 68 | 0.47 |
Cpn60β2 | 5,689 | 178 | 59 | 5,356 | 182 | 67 | 0.87 |
Cpn60β3 | 3,624 | 119 | 36 | 3,448 | 123 | 37 | 1.26 |
Cpn60β4 | 226b | 13b | 3b | 3,348 | 156 | 74 | 101 |
NdhJ | 57 | 2 | 15 | — | — | — | — |
NdhH | 44 | 2 | 4 | 614 | 24 | 53 | 43.4 |
a. emPAI Ratio means the ratio between emPAI score of proteins isolated from Cpn60β4 IP and Cpn60β1 IP fraction.
b. The sequences of these 13 peptides are identical between Cpn60β1 and Cpn60β4, suggesting that they are sequenced from Cpn60β1. RT-PCR showed that crr27-1 is a null mutant (Figure 1F).