Table 2.

Protein expression of Adiponectin, AdipoR1, AdipoR2, Leptin, ObR, ObRb in mammary tissue and Aiponectin and Leptin in Mammary Fat Pad of MMTV-TGF-α mice with and without mammary tumor; densitometry units (optical density of protein/optical density of β-actin)

Mammary tissue
	AL n=5	CCR n=5	ICR-Restricted n=5	ICR-Refed n=5
Adiponecin	1.00±0.03	0.99±0.07	0.88±0.13	0.95±0.01
AdipoR1	0.44±0.04†	0.47±0.05*,†	0.61±0.05*	0.56±0.01*,†
AdipoR2	0.46±0.03	0.56±0.05	0.53±0.03	0.47±0.00
Leptin	0.90±0.05*	0.60±0.03†	0.63±0.08†	0.73±0.03*,†
ObR	0.63±0.06*	0.58±0.13*,†	0.42±0.08*,†	0.26±0.04†
ObRb	0.79±0.10*	0.68±0.04*,†	0.53±0.03†	0.61±0.06*,†
Mammary tumor
	AL n=5	CCR n=5	ICR-Restricted n=3	ICR-Refed n=3
Adiponecin	0.75±0.12	0.53±0.06	0.55±0.15	0.63±0.01
AdipoR1	0.40±0.05	0.35±0.03	0.52±0.10	0.42±0.02
AdipoR2	0.53±0.07†	0.49±0.05†	0.76±0.03*	0.49±0.04†
Leptin	0.77±0.03	0.79±0.02	0.75±0.06	0.76±0.02
ObR	0.62±0.01	nd	0.42	nd
ObRb	0.55±0.05	nd	0.63	nd
Mammary Fat Pad (tumor-free mice)
	AL n=5	CCR n=5	ICR-Restricted n=5	ICR-Refed n=5
Adiponectin	0.73±0.08†	0.70±0.06†	1.00±0.03*	0.95±0.05*,†
Leptin	1.25±0.05	1.22±0.14	1.26±0.05	1.32±0.14
Mammary Fat Pad (tumor-bearing mice)
	AL n=5	CCR n=5	ICR-Restricted n=3	ICR-Refed n=3
Adiponectin	nd	nd	nd	1.12
Leptin	1.42±0.23	1.48±0.20	1.49±0.03	1.59±0.06

NOTE: Values are mean ± SE, n – number of probed samples

^{*, †}Values within a row with different superscript symbol are significantly different by ANOVA

nd – not detected