Fig. 5. HopF2 suppresses plant nonhost resistance.
A. HopF2 transgenic plants support non-adaptive bacteria growth. Four-week-old plants (Col-0 control, HopF2 or AvrPto transgenic plants) were sprayed with 10 µM DEX for 24 hr before bacterial inoculation. Arabidopsis leaves were inoculated with Pph NPS3121 (Pph) at 5 × 105 cfu/ml. The bacterial counting was performed 3 days after inoculation. * indicates a significant difference with p<0.05 when compared with data from control plants based on the results of an unpaired Student’s t-test.
B. RT-PCR analysis of MAMP marker gene induction. Ten-day-old seedlings were treated with 10 µM DEX for 24 hr, then treated with 10 nM flg22 for 0.5 and 1 hr.
C. Susceptibility to botrytis. Four-week-old plants were sprayed with 10 µM DEX for 24 hr before Botrytis cinerea strain BO5 were sprayed at concentration of 105 spores/ml. Disease symptom was recorded 2 days after infection.
D. Lesion development of B. cinerea infection. The area of infection was measured 4 days postinoculation. * indicates a significant difference with p<0.05.