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. Author manuscript; available in PMC: 2012 Mar 31.
Published in final edited form as: J Immunol Methods. 2011 Feb 4;367(1-2):1–16. doi: 10.1016/j.jim.2011.01.015

TABLE 2.

Stain Combinations to Study Human Malaria Parasite Growth and Invasion

Stain Interacts with Excitation
Maximum		 Emission
Maximum		 Dose Fixative Reference
Hydroethidine
+
Thiazole Orange 		DNA 536 610 158.48 μM None (Jouin et al., 2004)
Predominately
RNA		 509 533 100 nM1
Hoechst 33342
+
Thiazole Orange 		DNA Only 355 465 4 μM None (Grimberg et al., 2008)2, 4
Predominately
RNA		 509 533 100 nM
Hoechst 33342
+
Thiazole Orange
+
DiIC 1-5 		DNA Only 355 465 5 μM None (Grimberg et al., 2009)3, 4
Predominately
RNA		 509 533 100 nM
Membrane
Potential		 638 658 25 nM
1

Retic-Count Solution was later determined to contain 100 nM Thiazole Orange.

2

Also used by Jouin et al.(1995) using 32 μM Hoechst and 1 mL of BD Retic-Count solution 1.

3

Used to determine IC50 of antimalarial compounds.

4

Allows for differentiation of parasite erythrocytic stages (rings, trophozoites, schizonts).