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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: J Cell Biochem. 2011 May;112(5):1410–1417. doi: 10.1002/jcb.23057

FIGURE 2. Pax7-FKHR transcriptional activity is specifically enhanced by MyoD expression.

FIGURE 2

A. Pax7-FKHR-dependent activation of the Pax3/7 reporter gene 6xPRS9-luc, is increased ~2 fold upon co-expression with MyoD in C3H10T1/2 cells maintained in myoblast proliferation medium (GM). Note that MyoD has no detectable effect on Pax7 transcriptional activity. B. Pax7-FKHR activity is reduced close undetectable levels when transfected cells are maintained in myogenic differentiation medium (DM). However, robust Pax7-FKHR activity is observed upon MyoD co-expression (> 70 fold activation over Pax7-FKHR alone, Figure 2A). Pax7 activity is only mildly affected by MyoD. A-B, mean values are representative of at least three independent experiments. Error bars= standard deviation. C. Myogenin cannot recapitulate enhancement of Pax7-FKHR transcriptional activity in differentiating cells. As shown in B, MyoD co-expression is sufficient to maintain maximum Pax7-FKHR activity in myogenic differentiation cultures (compare with Figure 2B). Mean values where calculated from three independent experiments. Error bars= standard error of the mean.