Fig. 3.

Mitochondrial AIF level and PARP-1 activity in PC12 cells transfected with human APP. a The mitochondrial AIF protein level and its gene expression (Aifm1) in PC12 control cells and APPwt or APPsw. Protein level was estimated by western blot. AIF mRNA was evaluated using Real Time PCR. The results are means ± S.E.M. of 4 experiments normalized against β-actin. b PARP-1 activity was measured radiochemically with [¹⁴C] NAD⁺ [27] in PC12 cell lines. PARP-1 (Adpr1) mRNA was evaluated using Real Time PCR. The data represent the mean value ± S.E.M for 3 independent experiments (for PARP-1 activity in triplicate). *P < 0.05, Student`s t test