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. Author manuscript; available in PMC: 2011 Apr 6.
Published in final edited form as: Thromb Haemost. 2009 Jul;102(1):35–41. doi: 10.1160/TH08-12-0818

Figure 5. Inhibitory activity of anti-fVIII antibodies.

Figure 5

A. Supernatants from anti-human fVIII hybridomas (open circles) and anti-porcine fVIII hybridomas (gray circles) were analyzed for total anti-fVIII antibodies by ELISA and for inhibitory anti-fVIII antibodies by Bethesda assay (see “Materials and Methods”). The ordinate axis represents the ratio of the inhibitory activity (BU/ml) and ELISA reading (A405/ml). Numbers below the data points represent the numbers of hybridomas with no detectable inhibitory activity (BU/A405 < 0.4). Numbers above the data points represent the numbers of hybridomas with detectable inhibitory activity. B. Specific inhibitory activities of purified anti-human (open circles) and anti-porcine (gray circles) fVIII MAbs were determined using the Bethesda assay and the concentration of purified antibody.