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. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Int J Radiat Oncol Biol Phys. 2011 Jan 13;79(4):1206–1215. doi: 10.1016/j.ijrobp.2010.10.058

Figure 5.

Figure 5

(A) NFκB-DNA binding activity in SH-SY5Y and SK-N-MC cells either exposed to 2Gy, transiently transfected with NFκB (p50/p65 subunits) with or without curcumin or transfected with RelA siRNA and exposed to IR. (B) Identification of NFκB subunits by supershift assay. The addition of antibodies directed against potential components of NFκB complex resulted in supershift when antibodies of p50 or p65 were used. (C&D) Densitometry analysis showing inhibition of IR-induced NFκB DNA binding activity in RelA siRNA transfected cells and, curcumin associated complete suppression of NFκB-DNA binding activity in p50/p65 overexpressed (C) SH-SY5Y and (D) SK-N-MC cells. (E) Representative immunoblot showing robust induction of p50 and p65 protein levels in NFκB (p50, p65 subunits) overexpressed cells. α-tubulin was used to validate equal loading of samples.