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. Author manuscript; available in PMC: 2011 Apr 6.
Published in final edited form as: J Immunol. 2008 Dec 15;181(12):8237–8247. doi: 10.4049/jimmunol.181.12.8237

FIGURE 1.

FIGURE 1

Characterization of the expression of IL-15 on TC-1 cells transduced with IL-15 and/or IL-15RA. A, Schematic diagram of the IL-15 construct. TC-1 cells were transduced with a retrovirus encoding the IL2SP linked to IL-15 followed by IRES and GFP. This construct is denoted as IL-15. The IL-15-transduced TC-1 cells were sorted for GFP expression, and a portion of GFP+ cells was further transduced with retrovirus-encoding IL-15RA. Cells were analyzed for IL-15 or IL-15RA expression by flow cytometry analysis. B, Representative flow cytometry data demonstrating the expression of IL-15 and IL-15RA in cells transduced with IL-15 and/or IL-15RA. Note that surface expression of IL-15 was significantly higher on the TC-1 cells transduced with both IL-15 and IL-15RA. The data shown here are from one representative experiment of two performed.