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. Author manuscript; available in PMC: 2011 Apr 6.
Published in final edited form as: Oncogene. 2009 Mar 2;28(15):1782–1791. doi: 10.1038/onc.2009.16

Figure 2. Effect of RNase L over-expression and knockout on HuR and cellular growth.

Figure 2

(A) Equal amount of proteins extracted from confluent cells that stably express neomycin vector or RNase L/neomycin were analyzed by Western Blotting using anti-HuR and anti-β-actin. Data shown are two representative experiments of three. Lower panel showed quantitation of β-actin normalized signals (Mean ±SEM) of HuR from three independent experiments. ** denotes p<0.01 using student t-test statistic.

(B) Equal amount of proteins extracted from confluent MEFs generated from wild type or RNASEL-knockout mice were used for Western blotting (anti-mouse RNase L, anti-HuR and anti-β-actin). Right panel showed quantitation of β-actin normalized signals (Mean ± SEM) of HuR from three independent experiments. *** denotes p<0.001 using student t-test statistics.

(D) Growth curve of RNASEL-knockout and wild type MEFs. Cells were seeded in a 96-well plate with 5000 cells (upper panel) or 2500 cells per well (lower panel). Cellular growth monitoring and statistics were performed as described in (Fig. 1A).