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. Author manuscript; available in PMC: 2011 Apr 6.
Published in final edited form as: Oncogene. 2009 Mar 2;28(15):1782–1791. doi: 10.1038/onc.2009.16

Figure 3. Effect of RNase L on HuR mRNA.

Figure 3

(A) RNase L-/-and RNase L+/+ MEFs were treated with medium control (0 time point) or actinomycin D (5 ug/ml) for various times, as indicated. Semi-quantitative PCRs were performed with primers specific to HuR and β-actin cDNA. PCR, with cycle number that allows at least semi-quantitative comparison, was used as described Materials and Methods. Gels shown are from two independent experiments.

(B) Signal intensities on gels from Fig. 3A were quantified. One-phase exponential decay curves for relative mRNA half-life measurements in the two cell types are shown. Density units (minus background) are plotted as a function of time after the addition of actinomycin D. Detail of the model is given in Materials and Methods.