Table 1.
Immunohistochemical methods used in this study
| Antibody (Clone/Product code) | Dilution | Immunostaining method | Source of primary antibody |
|---|---|---|---|
| PRDX I | 1:1500 | Histostain-Plus Bulk Kit | Labfrontier, York, United Kingdom |
| PRDX II | 1:1000 | Histostain-Plus Bulk Kit | Labfrontier, York, United Kingdom |
| PRDX III | 1:500 | Histostain-Plus Bulk Kit | Labfrontier, York, United Kingdom |
| PRDX IV | 1:500 | Histostain-Plus Bulk Kit | Labfrontier, York, United Kingdom |
| PRDX V | 1:2000 | Histostain-Plus Bulk Kit | Labfrontier, York, United Kingdom |
| PRDX VI | 1:2000 | Histostain-Plus Bulk Kit | Labfrontier, York, United Kingdom |
| TRX | 1:200 | A biotinylated secondary anti-goat antibody; avidin-biotin-peroxidase complex | American Diagnostica, Greenwich, CT |
| 8-OHdG | 1:50 | Dako Envision Kit | JaICA, Fukuroi, Japan |
| Nrf2 | 1:100 | Histostain Plus Broad Spectrum Kit | Santa Cruz Biotechnology, Santa Cruz, USA |
| Keap1 | 1:100 | Biocare Medical HRP Polymer Kit | Santa Cruz |