Table 1. Summary of newly confirmed sRNAs in chromosome and virulence plasmid of Shigella flexneri strain 301.

sRNA genes	Adjacent genes	Stranda	Northern size	5′ endb	3′endb	Methodc
pssrA	CP0121/ipaJ	←→→	∼90	∼103842	103931	R/M/P
pssrB	virG/CP0183	→→→	∼200	∼152821	153020	R/M/P
cssrA	map/rpsB	←→→	∼110	∼181629	181738	R/M/P
cssrB	SF2021/SF2022	→←→	∼180	∼2046404	2046225	R/M/P
cssrC	SF2042/SF2043	←←←	∼340	∼2064237	2063898	R/M/P
cssrD	rpsP/ffh	←←←	∼200	∼2745060	2744861	R/M/P
cssrE	yggN/yggL	←←←	∼140	∼3043882	3043743	R/M/P
cssrF	dacB/yhbZ	→←←	∼290	∼3322880	3322591	R/M/P
cssrG	rbsB/rbsK	→→→	∼230	∼3946524	3946755	R/M/P

^aThe middle arrow represents the sRNA gene, while the flanking arrows indicate the orientation of the adjacent genes, respectively. Genes present on the strand given in the S. flexneri strain 301 genome database are indicated by (→), and genes present on the complementary strand are indicated by (←).

^bThe sRNA 3′ boundaries are from rho-independent terminator predictions. 5′ boundaries are calculated according to the 3′-ends and northern results.

^csRNAs were predicted based on different methods. R, RNAz prediction; M, tiling array hybridization; P: RT-PCR verification.