Figure 2. Chrysotile effects on the cell cycle and the percentage of cells in metaphase, anaphase and telophase in HK2 cells.

Cells treated with chrysotile and allowed to recover for 2, 4 or 8 days in fiber-free medium were analyzed by flow cytometry and by immunofluorescence. A) Histograms in linear (red) and log (colored) scales from flow cytometry show the effects of chysotile on the cell cycle, specifically an increase in the number of G2/M and hypertetraploid cells; B) chrysotile-treated cells recovered for 2 and 4 days show an increase in the number of cells in metaphase and a decrease in cells in anaphase and telophase compared with control cells (P<0.01 for 48 h and P<0.001 for 4 days).