Fig. 6.
Localization of GFP-labelled ER and the 175 kDa myosin in mitotic cells. In metaphase, some portions of ER (A) and 175 kDa myosin (B) were localized in the spindle region. Arrow indicates the position of chromosomes aligned in the mid plane. During phragmoplast assembly, ER (C, E, and G) and 175 kDa myosin (D, F, and H) were accumulated in the equatorial plane (triangles in C, E, and G) and around daughter nuclei (arrows in C, E, and G). Bright signals of ER (E and G) were also found in the centre of the equatorial plane, although weaker signals of 175 kDa myosin (long arrows in F and H) than those at the margin of the equatorial plane (F and H) were present. In the mature phragmoplast, ER (I) and 175 kDa myosin (J) were concentrated in the equatorial plane (triangle in I). During disorganization of the phragmoplast, ER (K) and the 175 kDa myosin (L) appeared to be diffused from the equatorial plane (triangle in K). The arrow in K indicates the position of daughter nuclei. The bar represents 20 μm.