Fig. 7.

Transmission electron micrographs of pollen development of bcmf2 transgenic Chinese cabbage. (A) The free microspore of bcmf2 transgenic plants was normal. (B) Loss of the cloverleaf pattern of the microspore at the early uninucleate pollen stage in bcmf2 transgenic plants. (C) The great extent of the thickening in intine within the germinal furrow of bcmf2 transgenic plants at the late uninucleate pollen stage. (D–F) The free microspore, early-stage uninucleate microspores, and late-stage uninucleate pollen of empty vector-transformed plants. (G−I) The uneven distribution of the germinal furrow and an abnormal thickening of the intine observed in bcmf2 transgenic plants from binucleate to mature pollen stage. (J−L) Development of the microspore of empty vector-transformed plants from the binucleate to mature pollen stage showing three evenly distributed germinal furrows. Gf, germinal furrow; In, intine; V, vacuole.