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Analysis of GalMBP ligands from L-428 cells with anti-Lewisx antibodies. Elution fractions from solubilized L-428 cells purified over a column of immobilized GalMBP were analyzed on 15% SDS-polyacrylamide gels followed by Coomassie blue staining or electroblotting onto nitrocellulose and probing with anti-Lewisx antibodies and radiolabelled GalMBP. The different relative intensities of some of the bands suggest that GalMBP and the antibodies bind differentially to glycans.
